human collagen type iii alpha 1 elisa kit  (Novus Biologicals)

Journal: Food Science & Nutrition

Article Title: Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts

doi: 10.1002/fsn3.2763

Figure Lengend Snippet: Effect of fermented Dendrobium officinale polysaccharides (FDOP) on extracellular (ECM) degradation in ultraviolet radiation (UVA)‐damaged human skin fibroblasts (HSF). Effects of FDOP and water‐extracted D. officinale polysaccharides (DOP) on the content of collagen type 1 (COL‐I) (a) and transcription expression (e); effects of FDOP and DOP on the content of collagen type III (COL‐III) (b) and transcription expression (f); effects of FDOP and DOP on the content of elastin (ELN) (c) and transcription expression (g); effects of FDOP and DOP on the content of hyaluronan (d). Each value is expressed as mean ±standard deviation. Three parallel experiments were performed in each group. The difference analysis between other experimental groups and the UVA model group is indicated by “*" (*: p < .05, **: p < .01, ***: p < .001). The difference analysis between DOP and FDOP is indicated by “#” (#: p < .05, ##: p < .01, ###: p < .001)

Article Snippet: Trans‐minimal essential medium (Trans‐MEM) reduced‐serum medium was purchased from Guangzhou Yufeng Biotechnology Co., Ltd. EasyScript ® one‐step genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis SuperMix reverse transcription kit (TransStart ® Top Green qPCR SuperMix kit) were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Total glutathione peroxidase (GSH‐px) assay kit with nicotinamide adenine dinucleotide phosphate (NADPH), lipid peroxidation malondialdehyde (MDA) assay kit, catalase assay kit, reactive oxygen species (ROS) detection kit, radioimmunoprecipitation assay (RIPA) lysis buffer, and Cell Counting Kit 8 were purchased from Biorigin (Beijing) Inc. Hyaluronan enzyme‐linked assay (ELISA) kit, human elastase ELISA kit, human collagen Type I ELISA kit, human collagen Type III ELISA kit, human total matrix metalloproteinase 1 (MMP‐1) ELISA kit, human total matrix metalloproteinase 3 (MMP‐3) ELISA kit, and human NRF2 ELISA kit were procured from Cusabio Biotech Co., Ltd.

Techniques: Expressing, Standard Deviation

Journal: Scientific reports

Article Title: Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion.

doi: 10.1038/s41598-021-86522-6

Figure Lengend Snippet: Figure 4. Changes in various ECM components in corneal myofibroblasts are dependent on mitochondrial ROS and NF-κB signaling. (A) RT qPCR on the mRNA expression of COL1A1, COL3A1, COL5A1 and lumican in corneal myofibroblasts (n = 3). (B) Secretion of pro-collagen I, collagen III, collagen V, and hydroxyproline content in supernatant collected 2 d after fibrosis induction (n = 3). (C) RT qPCR of the mRNA expression of COL1A1, COL3A1, COL5A1 and lumican in CD+ corneal myofibroblasts. Cells were treated with 10 µM mitoTEMPO for 2 d (n = 3). (D) Secretion of pro-collagen I, collagen III, collagen V, and hydroxyproline content in supernatant collected 2 d after fibrosis induction in CD+ myofibroblasts simultaneously treated with 10 µM mitoTEMPO (n = 3). (E) RT qPCR of the mRNA expression of COL1A1, COL3A1, COL5A1 and lumican in CD+ corneal myofibroblasts treated with 20 nM TPCA for 48 h (n = 3). (F) Secretion of pro-collagen I, collagen III, collagen V, and hydroxyproline content in supernatant collected 2 days after fibrosis induction in CD+ myofibroblasts simultaneously treated with 20 nM TPCA (n = 3). Values are means ± SD. n.s. (not significant); *P < 0.05, **P < 0.01.

Article Snippet: Secretion of IL-8 was assessed with Human CXCL/IL-8 DuoSet (R&D Systems, #DY208), pro-collagen I was assessed with Human Pro-Collagen I alpha 1 DuoSet ELISA (R&D Systems, #DY6220), collagen III with Human Collagen, type III, alpha 1 (COL3A1) ELISA kit (Cusabio, Houston, TX, USA, # CSB-E13446h) and collagen V with Human Collagen, type V, alpha 1 (COL5A1) ELISA kit (Cusabio, # CSB-E13447h) according to the manufacturer protocol.

Techniques: Quantitative RT-PCR, Expressing

Journal: Scientific Reports

Article Title: Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion

doi: 10.1038/s41598-021-86522-6

Figure Lengend Snippet: Changes in various ECM components in corneal myofibroblasts are dependent on mitochondrial ROS and NF-κB signaling. ( A ) RT qPCR on the mRNA expression of COL1A1, COL3A1, COL5A1 and lumican in corneal myofibroblasts ( n = 3). ( B ) Secretion of pro-collagen I, collagen III, collagen V, and hydroxyproline content in supernatant collected 2 d after fibrosis induction ( n = 3). ( C ) RT qPCR of the mRNA expression of COL1A1, COL3A1, COL5A1 and lumican in CD+ corneal myofibroblasts. Cells were treated with 10 µM mitoTEMPO for 2 d ( n = 3). ( D ) Secretion of pro-collagen I, collagen III, collagen V, and hydroxyproline content in supernatant collected 2 d after fibrosis induction in CD+ myofibroblasts simultaneously treated with 10 µM mitoTEMPO ( n = 3). ( E ) RT qPCR of the mRNA expression of COL1A1, COL3A1, COL5A1 and lumican in CD+ corneal myofibroblasts treated with 20 nM TPCA for 48 h ( n = 3). ( F ) Secretion of pro-collagen I, collagen III, collagen V, and hydroxyproline content in supernatant collected 2 days after fibrosis induction in CD+ myofibroblasts simultaneously treated with 20 nM TPCA ( n = 3). Values are means ± SD. n.s. (not significant); *P < 0.05, **P < 0.01.

Article Snippet: Secretion of IL-8 was assessed with Human CXCL/IL-8 DuoSet (R&D Systems, #DY208), pro-collagen I was assessed with Human Pro-Collagen I alpha 1 DuoSet ELISA (R&D Systems, #DY6220), collagen III with Human Collagen, type III, alpha 1 (COL3A1) ELISA kit (Cusabio, Houston, TX, USA, # CSB-E13446h) and collagen V with Human Collagen, type V, alpha 1 (COL5A1) ELISA kit (Cusabio, # CSB-E13447h) according to the manufacturer protocol.

Techniques: Quantitative RT-PCR, Expressing